Overview

In this jigsaw-based activity, students review protein separation methods. The class is divided into 2 groups, with about 3-5 students per group. One group reviews polyacrylamide gel electrophoresis (PAGE)-based protein separation, while the other group reviews liquid chromatography (LC)-based protein separation. Each group is responsible for looking up either PAGE or LC methods (4 methods per group), and each student within the group is responsible for learning all the material.
The class comes back together, and one student from each group is paired with a student from the opposite group. Pairs teach each other about the protein separation methods they ...

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In this jigsaw-based activity, students review protein separation methods. The class is divided into 2 groups, with about 3-5 students per group. One group reviews polyacrylamide gel electrophoresis (PAGE)-based protein separation, while the other group reviews liquid chromatography (LC)-based protein separation. Each group is responsible for looking up either PAGE or LC methods (4 methods per group), and each student within the group is responsible for learning all the material.
The class comes back together, and one student from each group is paired with a student from the opposite group. Pairs teach each other about the protein separation methods they reviewed.
The class regroups and the instructor goes over the material to make sure there are no misconceptions and to answer any student questions.

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Objectives

Students review/learn about two main types of protein separation methods.

Students work both as a team and individually during this activity, enabling them to develop autonomous critical thinking skills, as well as collaborative skills.

Context and requirements

Level University
Discipline Biology
Activity Content Protein separation methods
Technological Requirements None
Best Use Practice

Author’s Notes

Benefits

This is a time saving activity. Rather than lecture students on each individual separation method, students are responsible for learning it themselves in groups and teaching their peers. Student engagement and material retention have improved since using this activity to review protein separation methods in the Functional Genomics course.

Challenges

Making sure there are no misconceptions propagated through students teaching each other.

Tips

Review the material together as a class at the end of the activity to address any misconceptions that might have arisen from peer teaching.

Activity Pedagogical Components

Problem Solving

Students get into groups and review either PAGE or LC protein separation. Discuss among their group members about the different PAGE or LC methods.

Analysis

In pairs, students take turns teaching each other about PAGE or LC questions. Should know the material well enough to answer their classmates’ questions.

Review

Instructor reviews material with the entire class and answers student questions.

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