Polymerase Chain Reaction (PCR) is a powerful Molecular Biology tool in both research and diagnostics. Students will gain an insight into the use of PCR to screen for the Huntington disease (HTT gene) and how powerful PCR can be in amplifying many copies of DNA template. In conjunction to learning about PCR, students will also be learning about the mechanisms involved in DNA replication.
Students will apply the logic of PCR to help Delilah, a mother of 4 children and the daughter of a man with Huntington, try to understand whether she carries the CAG trinucleated repeat that causes the disease. This case study can span multiple weeks by having a lab component to complement the class activity.
This mini case study uses the flipped classroom strategy where students will prepare by watching videos, completing assigned readings and taking a quiz before entering the classroom. The instructor can use just in time teaching (JITT) by using the quiz results to alter the in-class material.
Within the classroom the students are split into groups and complete an assignment on Huntington disease. The assignment will be followed by some multiple choice clicker questions. The students will have applied their knowledge on PCR and gain a better understanding on DNA replication.
Students will be able to:
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